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KMID : 0880220120500050855
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Journal of Microbiology
2012 Volume.50 No. 5 p.855 ~ p.859
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Burkholderia denitrificans sp. nov., isolated from the soil of Dokdo Island, Korea
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Lee Chang-Muk
Yoon Sang-Hong
Koo Bon-Sung
Weon Hang-Yeon
Kim Soo-Jin
Kwon Soon-Wo
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Abstract
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A novel, Gram-negative, bacterial strain KIS30-44T was identified from wet forest soil collected on the Korean island of Dokdo. Growth of the strain was observed at 15?30°C, pH 5?9, 0?3% NaCl, and 950 mM KNO3. KIS30-44T reduced nitrate to nitrogen gas. Analysis of the 16S rRNA gene sequence showed that KIS30-44T was phylogenetically related to Burkholderia sacchari, Burkholderia mimosarum, and Burkholderia oxyphila (98.1%, 98.0%, and 98.0% sequence similarity, respectively). The genomic G+C content was 63.5 mol%. KIS30-44T exhibited less than 52% DNA-DNA relatedness with the type strains of 9 closely related Burkholderia species. The major isoprenoid quinone was Q-8. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and two unknown aminolipids. The major fatty acids in KIS30-44T were C16:0, C18:1ω7c and summed feature 3 (iso-C15:0 2-OH and C16:1ω7c), and the strain contained half the amount of C17:0 cyclo found in the 9 closely related Burkholderia species. The results of these phenotypic, 16S rRNA gene sequence, DNA-DNA hybridization, and chemotaxonomic data indicate that KIS30-44T represents a novel species within the genus Burkholderia, for which the name Burkholderia denitrificans (Type strain KIS30-44T =KACC 12733T =DSM 24336T) is proposed.
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KEYWORD
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Burkholderia denitrificans, 16S rRNA gene sequence, taxonomy
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